southern blotmedical dictionary

A technique used for searching for a specific DNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix DNA fragments

5. probe with labelled messenger RNA or cDNA

6. wash

Complementary mRNA/cDNA fragments will have hybridised.

(09 Oct 1997)