dictionary help| southern blot | medical dictionary |
A technique used for searching for a specific DNA fragment. The process is as follows:
1. separate DNA fragments by gel electrophoresis
2. Change pH of gel to basic, thus allowing disruption of H-bonds
3. blot gel with nitrocellulose paper
4. Heat paper so as to fix DNA fragments
5. probe with labelled messenger RNA or cDNA
6. wash
Complementary mRNA/cDNA fragments will have hybridised.
(09 Oct 1997)
southcottian, southdown, southeastern United States < Prev | Next > Southern blot analysis, Southern blotting
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| Southern blot analysis | medical dictionary |
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes.
(05 Mar 2000)
southdown, southeastern United States, southern blot < Prev | Next > Southern blotting, Southern, M.E., southernwood
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| Southern blotting | medical dictionary |
Preferred term: blotting
southeastern United States, southern blot, Southern blot analysis < Prev | Next > Southern, M.E., southernwood, Southey, Reginald
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| Southern, M.E. | medical dictionary |
southern blot, Southern blot analysis, Southern blotting < Prev | Next > southernwood, Southey, Reginald
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| southernwood | medical dictionary |
<botany> A shrubby species of wormwood (Artemisia Abrotanum) having aromatic foliage. It is sometimes used in making beer.
(01 Mar 1998)
Southern blot analysis, Southern blotting, Southern, M.E. < Prev | Next > Southey, Reginald, Southey's tubes, southing
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