|mutagenesis, site-directed||medical dictionary|
Mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule. The most common method involves use of a chemically synthesised oligonucleotide mutant which can hybridise with the DNA target molecule. The resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.
(12 Dec 1998)
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