bandshift assaymedical dictionary

<investigation>

An assay for proteins, such as transcription factors, that bind specific DNA sequences.

A labelled oligonucleotide corresponding to the recognition sequence is incubated with an appropriate nuclear protein extract and run on a nondenaturing acrylamide gel. Oligonucleotides that have been bound by proteins are retarded relative to those that are unbound.

This entry appears with permission from the Dictionary of Cell and Molecular Biology

(11 Mar 2008)