southern blotmedical dictionary

A technique used for searching for a specific DNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix DNA fragments

5. probe with labelled messenger RNA or cDNA

6. wash

Complementary mRNA/cDNA fragments will have hybridised.

(09 Oct 1997)

Southern blot analysismedical dictionary

A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes.

(05 Mar 2000)

Southern blottingmedical dictionary

Preferred term: blotting

Southern, M.E.medical dictionary

<person>

A 20th century British biologist.

See: Southern blot analysis.

(05 Mar 2000)

southern blot, Southern blot analysis, Southern blotting < Prev | Next > southernwood, Southey, Reginald

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southernwoodmedical dictionary

<botany> A shrubby species of wormwood (Artemisia Abrotanum) having aromatic foliage. It is sometimes used in making beer.

(01 Mar 1998)